rabbit polyclonal anti runt related transcription factor 2  (Boster Bio)

Journal: ACS applied materials & interfaces

Article Title: Immunomodulatory ECM-like Microspheres for Accelerated Bone Regeneration in Diabetes Mellitus

doi: 10.1021/acsami.7b18458

Figure Lengend Snippet: Expression of Osterix and Runx2 in the defect area 14 days after the surgical operation. (A, B) Immunohistochemical images showed significantly more Osterix+ and Runx2+ osteoprogenitor cells in normal control and IL4-loaded NHG-MS DM groups than in the other two DM groups. (C–F) Quantitative analysis of the number of Osterix+ and Runx2+ cells and mean integral optical density (IOD) (*P < 0.05, **P < 0.01).

Article Snippet: Antibodies used included rabbit polyclonal anti-TNF- α (1:200, ab6671, Abcam), rabbit polyclonal anti-Runt-related transcription factor 2 (Runx2, 1:1000, ab23981, Abcam), and anti-Osterix (1:400, ab22552, Abcam).

Techniques: Expressing, Immunohistochemical staining

Journal: International journal of molecular medicine

Article Title: Expression of PADI4 in patients with ankylosing spondylitis and its role in mediating the effects of TNF-α on the proliferation and osteogenic differentiation of human mesenchymal stem cells.

doi: 10.3892/ijmm.2015.2248

Figure Lengend Snippet: Figure 8. Western blot analysis of bone morphogenetic protein 2 (BMP-2), runt- related transcription factor 2 (Runx) and Osterix protein expression in human mesenchymal stem cells (hMSCs) following transfection with peptidyl arginine deiminase, type IV (PADI4) siRNA in the presence or absence of tumor necrosis factor-α (TNF-α). (A) Blots showing BMP-2, Runx2, Osterix and β-actin protein expression. Lane 1, vehicle-treated group; lane 2, vehicle + PADI4 siRNA-treated group; lane 3, TNF-α-treated group; lane 4, TNF-α + PADI4 siRNA-treated group. (B) Relative protein expression of BMP-2. (C) Relative protein expression of Runx2. (D) Relative protein expression of Osterix. β-actin was used as a loading control. *P<0.05 compared with the vehicle-treated group. **P<0.05 compared with the TNF-α-treated group.

Article Snippet: Following blocking with 5% non-fat milk, the membranes were incubated with anti-PADI4 rabbit polyclonal antibody (Cat. no. sc-98991, 1:500 dilution; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-bone morphogenetic protein 2 (BMP-2) mouse monoclonal antibody (Cat. no. ab6285, 1:400 dilution), anti runt-related transcription factor 2 (Runx2) rabbit polyclonal antibody (Cat. no. ab102711, 1:400 dilution), anti-Osterix mouse monoclonal antibody (Cat. no. ab57335, 1:800 dilution) (all from Abcam, Cambridge, MA, USA) and anti β-actin mouse monoclonal antibody (Cat. no. BM0627, 1:1,000 dilution; Boster, Wuhan, China) at 37 ̊C for 2 h. The membranes were washed 3 times with TBST and incubated with rabbitanti mouse IgG (Cat. no. sc-358913, 1:2,000 dilution) or mouse-anti rabbit IgG (Cat. no. sc-2357, 1:2,000 dilution) horseradish peroxidase (HRP)-conjugated secondary antibody (both from Santa Cruz Biotechnology) at 37 ̊C for 1 h. The signals were detected using an ECL detection kit (Pierce Biotechnology, Inc.).

Techniques: Western Blot, Expressing, Transfection, Control